In Vivo Selection of H1N2 Influenza Virus Reassortants in the Ferret Model
Identifieur interne : 000927 ( Main/Exploration ); précédent : 000926; suivant : 000928In Vivo Selection of H1N2 Influenza Virus Reassortants in the Ferret Model
Auteurs : Matthew Angel [États-Unis] ; J. Brian Kimble [États-Unis] ; Lindomar Pena [États-Unis] ; Hongquan Wan [États-Unis] ; Daniel R. Perez [États-Unis]Source :
- Journal of Virology [ 0022-538X ] ; 2013.
Abstract
Although the ferret model has been extensively used to study pathogenesis and transmission of influenza viruses, little has been done to determine whether ferrets are a good surrogate animal model to study influenza virus reassortment. It has been previously shown that the pandemic 2009 H1N1 (H1N1pdm) virus was able to transmit efficiently in ferrets. In coinfection studies with either seasonal H1N1 or H3N2 strains (H1N1s or H3N2s, respectively), the H1N1pdm virus was able to outcompete these strains and become the dominant transmissible virus. However, lack of reassortment could have been the result of differences in the cell or tissue tropism of these viruses in the ferret. To address this issue, we performed coinfection studies with recombinant influenza viruses carrying the surface genes of a seasonal H3N2 strain in the background of an H1N1pdm strain and vice versa. After serial passages in ferrets, a dominant H1N2 virus population was obtained with a constellation of gene segments, most of which, except for the neuraminidase (NA) and PB1 segments, were from the H1N1pdm strain. Our studies suggest that ferrets recapitulate influenza virus reassortment events. The H1N2 virus generated through this process resembles similar viruses that are emerging in nature, particularly in pigs.
Url:
DOI: 10.1128/JVI.02591-12
PubMed: 23302886
PubMed Central: 3592131
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000662
- to stream Pmc, to step Curation: 000662
- to stream Pmc, to step Checkpoint: 000660
- to stream Ncbi, to step Merge: 000859
- to stream Ncbi, to step Curation: 000859
- to stream Ncbi, to step Checkpoint: 000859
- to stream Main, to step Merge: 000928
- to stream Main, to step Curation: 000927
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en"><italic>In Vivo</italic> Selection of H1N2 Influenza Virus Reassortants in the Ferret Model</title><author><name sortKey="Angel, Matthew" sort="Angel, Matthew" uniqKey="Angel M" first="Matthew" last="Angel">Matthew Angel</name><affiliation wicri:level="4"><nlm:aff id="aff1">Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region><settlement type="city">College Park (Maryland)</settlement></placeName><orgName type="university">Université du Maryland</orgName></affiliation></author><author><name sortKey="Kimble, J Brian" sort="Kimble, J Brian" uniqKey="Kimble J" first="J. Brian" last="Kimble">J. Brian Kimble</name><affiliation wicri:level="4"><nlm:aff id="aff1">Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region><settlement type="city">College Park (Maryland)</settlement></placeName><orgName type="university">Université du Maryland</orgName></affiliation></author><author><name sortKey="Pena, Lindomar" sort="Pena, Lindomar" uniqKey="Pena L" first="Lindomar" last="Pena">Lindomar Pena</name><affiliation wicri:level="4"><nlm:aff id="aff1">Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region><settlement type="city">College Park (Maryland)</settlement></placeName><orgName type="university">Université du Maryland</orgName></affiliation></author><author><name sortKey="Wan, Hongquan" sort="Wan, Hongquan" uniqKey="Wan H" first="Hongquan" last="Wan">Hongquan Wan</name><affiliation wicri:level="2"><nlm:aff id="aff2">Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Perez, Daniel R" sort="Perez, Daniel R" uniqKey="Perez D" first="Daniel R." last="Perez">Daniel R. Perez</name><affiliation wicri:level="4"><nlm:aff id="aff1">Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region><settlement type="city">College Park (Maryland)</settlement></placeName><orgName type="university">Université du Maryland</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23302886</idno><idno type="pmc">3592131</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592131</idno><idno type="RBID">PMC:3592131</idno><idno type="doi">10.1128/JVI.02591-12</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000662</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000662</idno><idno type="wicri:Area/Pmc/Curation">000662</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000662</idno><idno type="wicri:Area/Pmc/Checkpoint">000660</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000660</idno><idno type="wicri:Area/Ncbi/Merge">000859</idno><idno type="wicri:Area/Ncbi/Curation">000859</idno><idno type="wicri:Area/Ncbi/Checkpoint">000859</idno><idno type="wicri:Area/Main/Merge">000928</idno><idno type="wicri:Area/Main/Curation">000927</idno><idno type="wicri:Area/Main/Exploration">000927</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>In Vivo</italic> Selection of H1N2 Influenza Virus Reassortants in the Ferret Model</title><author><name sortKey="Angel, Matthew" sort="Angel, Matthew" uniqKey="Angel M" first="Matthew" last="Angel">Matthew Angel</name><affiliation wicri:level="4"><nlm:aff id="aff1">Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region><settlement type="city">College Park (Maryland)</settlement></placeName><orgName type="university">Université du Maryland</orgName></affiliation></author><author><name sortKey="Kimble, J Brian" sort="Kimble, J Brian" uniqKey="Kimble J" first="J. Brian" last="Kimble">J. Brian Kimble</name><affiliation wicri:level="4"><nlm:aff id="aff1">Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region><settlement type="city">College Park (Maryland)</settlement></placeName><orgName type="university">Université du Maryland</orgName></affiliation></author><author><name sortKey="Pena, Lindomar" sort="Pena, Lindomar" uniqKey="Pena L" first="Lindomar" last="Pena">Lindomar Pena</name><affiliation wicri:level="4"><nlm:aff id="aff1">Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region><settlement type="city">College Park (Maryland)</settlement></placeName><orgName type="university">Université du Maryland</orgName></affiliation></author><author><name sortKey="Wan, Hongquan" sort="Wan, Hongquan" uniqKey="Wan H" first="Hongquan" last="Wan">Hongquan Wan</name><affiliation wicri:level="2"><nlm:aff id="aff2">Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Perez, Daniel R" sort="Perez, Daniel R" uniqKey="Perez D" first="Daniel R." last="Perez">Daniel R. Perez</name><affiliation wicri:level="4"><nlm:aff id="aff1">Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Virginia-Maryland Regional College of Veterinary Medicine, Department of Veterinary Medicine, University of Maryland, College Park, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region><settlement type="city">College Park (Maryland)</settlement></placeName><orgName type="university">Université du Maryland</orgName></affiliation></author></analytic><series><title level="j">Journal of Virology</title><idno type="ISSN">0022-538X</idno><idno type="eISSN">1098-5514</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Although the ferret model has been extensively used to study pathogenesis and transmission of influenza viruses, little has been done to determine whether ferrets are a good surrogate animal model to study influenza virus reassortment. It has been previously shown that the pandemic 2009 H1N1 (H1N1pdm) virus was able to transmit efficiently in ferrets. In coinfection studies with either seasonal H1N1 or H3N2 strains (H1N1s or H3N2s, respectively), the H1N1pdm virus was able to outcompete these strains and become the dominant transmissible virus. However, lack of reassortment could have been the result of differences in the cell or tissue tropism of these viruses in the ferret. To address this issue, we performed coinfection studies with recombinant influenza viruses carrying the surface genes of a seasonal H3N2 strain in the background of an H1N1pdm strain and vice versa. After serial passages in ferrets, a dominant H1N2 virus population was obtained with a constellation of gene segments, most of which, except for the neuraminidase (NA) and PB1 segments, were from the H1N1pdm strain. Our studies suggest that ferrets recapitulate influenza virus reassortment events. The H1N2 virus generated through this process resembles similar viruses that are emerging in nature, particularly in pigs.</p></div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region><settlement><li>College Park (Maryland)</li></settlement><orgName><li>Université du Maryland</li></orgName></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Angel, Matthew" sort="Angel, Matthew" uniqKey="Angel M" first="Matthew" last="Angel">Matthew Angel</name></region><name sortKey="Kimble, J Brian" sort="Kimble, J Brian" uniqKey="Kimble J" first="J. Brian" last="Kimble">J. Brian Kimble</name><name sortKey="Pena, Lindomar" sort="Pena, Lindomar" uniqKey="Pena L" first="Lindomar" last="Pena">Lindomar Pena</name><name sortKey="Perez, Daniel R" sort="Perez, Daniel R" uniqKey="Perez D" first="Daniel R." last="Perez">Daniel R. Perez</name><name sortKey="Wan, Hongquan" sort="Wan, Hongquan" uniqKey="Wan H" first="Hongquan" last="Wan">Hongquan Wan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000927 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000927 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:3592131
|texte= In Vivo Selection of H1N2 Influenza Virus Reassortants in the Ferret Model
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:23302886" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |